Juniper Publishers: Distribution of Coastal and Marine Herpetofauna in...

Juniper Publishers: Distribution of Coastal and Marine Herpetofauna in...: Oceanography & Fisheries Open AccessJournal Juniper Publishers Authored by Sandeep Munjpara* Abstract The Gulf of Kachchh ...

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SDS-PAGE Electrophoresis of Ocypode rotundata Miers, 1882 and O. ceratophthalma Pallas, 1772 using Muscle Tissue along the Coast of Pakistan

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  Oceanography & Fisheries Open Access Journal Juniper Publishers Authored by Sahir Odhano Abstract Electrophoretic studies for the identification of ghost crabs (Ocypode rotundata and Ocypode ceratophthalma) using the SDS-PAGE were performed on protein patterns along the Sandspit coastal areas. The muscle tissues were used to estimate the molecular mass of proteins through SDS-PAGE electrophoresis. Electrophoretic settings were standardized to use 10% acrylamide resolving gel and 5% acrylamide stacking gel. A discontinuous buffer system was used following the protocols of Laemmli (1970) to observe the relative mobility and molecular weight of proteins. In O. rotundata total of 6 protein bands were resolved among them band-5 was found similar in size to the marker used (BSA = 66 kDa MW). Whereas in O. ceratophthalma total of 8 protein bands were resolved among them three protein bands (band-6, band-7, and band-8) were found to be higher in size than the mole...
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